NEPHELOMETRY
This is another technology which works on the principle of immunoassay and is widely utilized in diagnostics. The simple and easy to work with automation of the analyzers under this principle makes it a favorable platform to work with.
PRINCIPLE OF NEPHELOMETRY
This technology is popularly used for quantification of immunoglobulin concentration in the sera of patients. During reaction, antigen-antibody complexes are generated in solution which is then quantified with the aid of a light beam. The light when incident on the immune complex, is scattered which is captured by lens fit at right angles to the incident light. This scattered light is then measured to determine concentration of the analyte of interest. This is popularly used for estimation of immunoglobulins like IgG, IgM, IgA, etc. which aids in diagnosis of allergy and autoimmune disorders.
This technology is popularly used for quantification of immunoglobulin concentration in the sera of patients. During reaction, antigen-antibody complexes are generated in solution which is then quantified with the aid of a light beam. The light when incident on the immune complex, is scattered which is captured by lens fit at right angles to the incident light. This scattered light is then measured to determine concentration of the analyte of interest. This is popularly used for estimation of immunoglobulins like IgG, IgM, IgA, etc. which aids in diagnosis of allergy and autoimmune disorders.
With the ability to quantify particulate immune complexes, the analyzers under this principle have become an important inclusion in diagnostic laboratories. The analyzer which works under this principle in our floor is BN II [Siemens] and IMMAGE® 800 [Beckman Coulter].